Distance Learning: Techniques in Structural Molecular Biology (TSMB)

Postgraduate Certificate

TSMB Syllabus

Section 1	Section 2	Section 3	Section 4	Section 5	Section 6
Section 7	Section 8	Section 9	Section 10	Section 11	Section 12

Section 11: Protein-Protein Interactions

This section examines the importance of protein-protein interactions, and explains methods for investigating them experimentally.

Introduction
Types of protein-protein interactions

Protease-Inhibitor and Antibody-Antigen Complexes
Molecular Machines
Structure of RNA polymerase II
Ribosome Structure
Structure of the Gamma complex
Structure of the Arp2/3 complex
Protein Modules Implicated in Protein-Protein Complexes
SH2 and PTB domains
SH3 and WW domains
Bromodomains
PDZ domains

The Growth Hormone Receptor and the "Hot Spot" Concept
Techniques to study protein-protein interactions

Pull-down of protein complexes
Mass spectrometry
Yeast 2-hybrid methods
Analytical Ultracentrifugation
Surface Plasmon Resonance
Calorimetry
Fluorescence Spectroscopy

Bioinformatics of protein-protein interactions
Protein-Protein Interactions and Drug Design

Objectives

To make students aware of methods for studying protein-protein interactions and their biophysical basis. At the end of this section, students should:

Appreciate the range of macromolecular complexes in nature.
To be familiar with various approaches for studying protein-protein interactions