Charge Distribution of T4 lysozyme in Molecular Surface T4 lysozyme experiment
The four attempts to introduce salt bridges on the surface of T4 lysozyme are summarized below. In each case a glutamic or aspartic acid was introduced close to an existing lysine or arginine. The crystal structures of the three glutamate containing mutants were deterined and found to be extremely similar to wild type.
Charge Distribution of T4 lysozyme in Molecular Surface 
Mutations designed to introduce salt bridges on the surfaces of T4 lysozyme