Postgraduate Certificate:
Protein Crystallography

Prospective students

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Related links

bullet MSc structural biology
bullet PPS course
bullet PX course
bullet TSMB course
bullet Commonwealth
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Current PX students
[Restricted access]

bullet 2009/10 course access
bullet 2008/9 course access

 

Protein Crystallography (PX)

Postgraduate Certificate

PX Syllabus

Section 9 - Molecular Replacement
  • Introduction
  • Background

Molecular Replacement Using Known Structures
Prerequisites
Patterson Function
Patterson Function and Molecular Replacement
Molecular Replacement by Rotation and Translation of a Model Patterson over the Target Patterson
NonCrystallographic Symmetry (NCS)

  • Steps and Strategy Using Known Structures

Model
Rotation Function

Eulerian Angles
Choice of Radius of Integration and Model Unit Cell
Non-Crystallographic Symmetry and SelfRotation
Polar Angles
Variable Parameters

Patterson Correlation Refinement

Eulerian Angles
Choice of Radius of Integration and Model Unit Cell
Non-Crystallographic Symmetry and SelfRotation
Polar Angles
Variable Parameters

Patterson Correlation Refinement
Translation Function

NonCrystallographic Symmetry

Rigid Body Refinement
Molecular Replacement Solution Checks
Statistics
Molecular Replacement Programs

  • Molecular Replacement Practice: Structure determination of a protein-DNA complex