Postgraduate Certificate:
Protein Crystallography

Prospective students

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Related links

bullet MSc structural biology
bullet PPS course
bullet PX course
bullet TSMB course
bullet Commonwealth
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Current PX students
[Restricted access]

bullet 2009/10 course access
bullet 2008/9 course access

 

Protein Crystallography (PX)

Postgraduate Certificate

PX Syllabus

Section 8 - Data Collection and Cryocrystallography
  • Introduction
  • Data Collection

Oscillation Geometry

Overview
Ewald Construction
Lunes
FrameOscillation Diffraction Pattern
Oscillation Angle
Fully and Partially Recorded Reflections
FrameIndexed Oscillation Pattern
Blind Region
Completeness

Steps and Strategy

Mounting Crystal in Capillary Tube
Centring Crystal on Oscillation Camera
Diffraction Quality
Rotation Axis and Angle

Diffraction Symmetry and Data Collection Strategy

Oscillation Angle
Oscillation Angle and Overlap
Efficient Data Collection Strategies

  • Cryocrystallography

Background

Cryocrystallography
Thermal Motion and Disorder
Radiation Damage

Steps and Strategy

Cryostats
Cryoprotectants
Handling and Mounting
FrameCooling the Crystal
FrameMounting the Crystal

Case Study - Gamma-B Crystallin C-Terminal Domain