Protein Crystallography (PX)

• Introduction
• Statistics

Population Mean and Standard Deviation
The Normal Distribution

• Data Processing

Steps and Strategy

Detector Calibration
Autoindexing, Integration, Correction and Reduction

Oscillation Geometry
Processing Geometry
Processing Parameters
Integration - Summation and Profile Fitting
Geometric Corrections

Scaling and Merging
Truncation
Missing Reflections
Processing and Merging Programs
Statistics

• Space Group Determination

Space Group Determination in Outline

Laue Symmetry
Reflection Conditions and Systematic Absences

Lattice Reflection Conditions
Screw Axis Reflection Conditions

Space Group Determination for the Enantiomorphic Space Groups
Space Group Determination Example
Space Group Determination in Practice

Initial Analysis

Bravais Lattice
Laue Class
Crystal Class
Screw Axes
Procedure

Further Analysis

Translation Vectors, Anomalous Dispersion, Derivative Patterson
Final

Pitfalls

Twinning
Incorrect Determination of Reflection Conditions
Pseudo Symmetry

Solvent Content of Protein Crystals and NCS