Protein Crystallography (PX)

Postgraduate Certificate

PX Syllabus

Section 1	Section 2	Section 3	Section 4	Section 5	Section 6	Section 7	Section 8
Section 9	Section 10	Section 11	Section 12	Section 13	Section 14	Section 15	Section 16

Section 10 - Isomorphous Replacement

Introduction
Background

Multiple Isomorphous Replacement and Anomalous Scattering [MIR(AS)]
Phase Determination by MIR
Probability Density Function (PDF)
Patterson Function
Superposition Function

Heavy Metal Derivatives

Heavy Metal Reagents
Soaking Parameters
Soaking Crystals
Isomorphism and Substitution

Steps and Strategy

Scaling

Scaling Programs

Patterson Search for Heavy Atom Sites

Symmorphic Space Groups - Patterson Symmetry
Patterson Search Programs

Refinement of Heavy Atom Parameters and Phasing

Refinement and Phasing Programs

Statistics