Protocol 3


Methionine pathway inhibition


  1. Spin down the cells form 1 ml of overnight culture in LB medium.
  2. Resuspend pellet in 1 ml of M9 medium [...] with carbon source (4 g/liter) and then add to 1 liter of the some prewarmed medium.
  3. Cells grow up to mid-log phase.
  4. At mid-log phase add amino acids: lysine, phenylalanine and threonine at 100 mg/liter; isoleucine and valine at 50 mg/liter; and selenomethionine at 60 mg/liter.
  5. Induce the culture 15 minutes after addition of the amino acids.


Back[Back] Index[Index] PPS[PPS]
Maciej Kozak's PPS'97 project
Protein engineering and its role in solving the phase problem
last updated