| Table; | caspase-1 | ,-2 | ,-3 | ,-4 | ,-5 | ,-6 | ,-7 | ,-8 | ,-9 | ,-10 |
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Sequence alignment of the caspases. Phylogenetic relationships were determined and polypeptide sequences for the human forms of the caspases aligned using the PileUp algorithm (Gentics Computer Group, Madison, WI, USA). The relationships are based on the full-length proenzymes. The dendrogram is not affected if the same analysis is carried out using caspases without their prodomains. Amino acid residues are numbered to the right of each sequence. The QACXG motif is conserved in all family members.
The underlined sequences represent the FADD/MORT1 homology domains in caspase-8 and caspase-9. For both caspase-2, -6 the largest isoforms were used.
(caspase-1,404 aa; caspase-2,435 aa; caspase-3,277 aa; caspase-4,377 aa; caspase-5,418 aa; caspase-6,293 aa; caspase-7,303 aa; caspase-8,479 aa; caspase-9,416 aa; caspase-10,479 aa, gap creation penalty = 5 and gap extension penalty = 3 ) |
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caspase-4 : ......................................................... : - caspase-5 : ......................................................... : - caspase-1 : ......................................................... : - caspase-3 : ......................................................... : - caspase-7 : ......................................................... : - caspase-6 : ......................................................... : - caspase-8 : .................MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKD : 40 caspase-10: MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSS : 57 caspase-2 : ......................................................... : - caspase-9 : ......................................................... : -
caspase-4 : ................................................MAEGNHRKK : 9 caspase-5 : ......MFKGILQSGLDNFVINHMLKNNVAGQTSIQTLVPNTDQKSTSVKKDNHKKK : 51 caspase-1 : ................................................MADKVLKEK : 9 caspase-3 : ......................................................... : - caspase-7 : ......................................................... : - caspase-6 : ......................................................... : - caspase-8 : ALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQ : 97 caspase-10: ASDVFEHLLAEDLLSEEDPFFLAELLY-IIRQKKLLQHLNCTKEEVERLL--PTRQR : 111 caspase-2 : ............MAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLE : 45 caspase-9 : ..........................MDEADRRLLRRCRLRLVEELQVDQLWDVLLS : 31
caspase-4 : PLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQR : 66 caspase-5 : TVKMLEYLGKDVLHGVFNYLAKHDVLTLKEEEKKKYYDAKIEDKALILVDSLR-KNR : 107 caspase-1 : RKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGA : 66 caspase-3 : ......................................................... : - caspase-7 : ......................................................... : - caspase-6 : ......................................................... : - caspase-8 : ISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGE : 154 caspase-10: VSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEM-TSLSFL-AFLEKQGKI--DE : 164 caspase-2 : KDIITLEMRELIQ-AKVGS-FSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDM : 100 caspase-9 : RELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASF : 88
caspase-4 : MAGQMLL-------------------QT---FFNI-D-QISPNKKAHPN-MEAGP-- : 96 caspase-5 : VAHQMFT-------------------QT---LLNM-D-QKITSVKPLLQ-IEAGP-- : 137 caspase-1 : QACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQDSQGVLSSFPAPQAVQDNPAM : 123 caspase-3 : .......................................MENTENSVDSKSIKNLEP : 18 caspase-7 : ...........MADDQGCIEEQGVEDSANEDSVDAK-PDRSSFVPSLFSKKKKNVTM : 45 caspase-6 : ...................................M-SSASGLRRGHPAGGEENMTE : 21 caspase-8 : GKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLE-GSPDEFSNGEELCG-VMTIS : 209 caspase-10: DNLTCLEDLCKTVVPKLLRNIEKY---KREKAIQIV-TPPVD-KEAESYQGEEELVS : 216 caspase-2 : LLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKK-LRLSTDTVEHSLDNKDGPVC : 156 caspase-9 : LRTNRQAGKLSKPTLE---NLT---PVVLR-PEIRK-PEVLRPETPRPVDIGSGGFG : 137
caspase-4 : PESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRN : 153 caspase-5 : PESAESTNILKLCPREEFLRLCKKNHDEIYPIKKREDRRRLALIICNTKFDHLPARN : 194 caspase-1 : PTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRT : 180 caspase-3 : KIIHGSESMDSGISLDNSYKM-DYPEMGLCIIINNKNFHKST---------GMTSRS : 65 caspase-7 : RSI--KTTRDR-VP-TYQYNM-NFEKLGKCIIINNKNFDKVT---------GMGVRN : 88 caspase-6 : TDAFYKREM---FDPAEKYKM-DHRRRGIALIFNHERFFWHL---------TLPERR : 65 caspase-8 : DS--PREQDSESQTLDKVYQM-KSKPRGYCLIINNHNFAKAREKVPKLH--SIRDRN : 261 caspase-10: QT--DVKTFLEALPRAAVYRM-NRNHRGLCVIVNNHSFT------------SLKDRQ : 258 caspase-2 : LQVKPCTPEFYQTHFQLAYRL-QSRPRGLALVLSNVHF-------TGEK--ELEFRS : 203 caspase-9 : -DVGAL--ESLRGNADLAYIL-SMEPCGHCLIINNVNF-------CRES--GLRTRT : 181
caspase-4 : GADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSH : 210 caspase-5 : GAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSH : 251 caspase-1 : GAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSH : 237 caspase-3 : GTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVS-KEDHSKRSSFVCVLLSH : 121 caspase-7 : GTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKAS-EEDHTNAACFACILLSH : 144 caspase-6 : RTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVS-TVSHADADCFVCVFLSH : 121 caspase-8 : GTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMD-HSNMDCFICCILSH : 317 caspase-10: GTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTH : 315 caspase-2 : GGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSH : 260 caspase-9 : GSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAR-QDHGALDCCVVVILSH : 237
caspase-4 : GILEGICGTVHDEKKPD--VLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGAN--R : 263 caspase-5 : GILEGICGTAHKKKKPD--VLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEK--H : 304 caspase-1 : GIREGICGKKHSEQVPD--ILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDS--P : 290 caspase-3 : G-------EEGIIFGTNGP-VDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDC : 170 caspase-7 : G-------EENVIYGKDGV-TPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDD : 193 caspase-6 : G-------EGNHIYAYDAK-IEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDV : 170 caspase-8 : G-------DKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQK : 367 caspase-10: G-------RFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQP : 365 caspase-2 : GVE-------GAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDR : 310 caspase-9 : GCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDH : 294
caspase-4 : GELWVRDSPASLEVASSQSSE----------NLEEDAVYKTHVEKDFIAFCSSTPHN : 310 caspase-5 : GELWVRDSPASLAVISSQSSE----------NLEADSVCKIHEEKDFIAFCSSTPHN : 351 caspase-1 : GVVWFKDSVGVSGNLSLPTTE----------EFEDDAIKKAHIEKDFIAFCSSTPDN : 337 caspase-3 : GI--------ETD--SG-VDDDMA--C-----------HKIPVDADFLYAYSTAPGY : 203 caspase-7 : AI--------QAD--SGPINDTDANPR-----------YKIPVEADFLFAYSTVPGY : 229 caspase-6 : PVIPLDVVDNQTEKLDTNITEVDAASV-----------YTLPAGADFLMCYSVAEGY : 216 caspase-8 : GI----PVETDSEEQPYLEMDLSSPQT-----------RYIPDEADFLLGMATVNNC : 409 caspase-10: SV----SIEADA-LNP--EQAPTSLQD-----------S-IPAEADFLLGLATVPGY : 403 caspase-2 : GVDQQDGKNHAGSPGCE-ESDA-----GK---EKLPKM-RLPTRSDMICGYACLKGT : 357 caspase-9 : GFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGF : 351
caspase-4 : VSWRDSTMGSIFITQLITCFQKYSWCC-HLEEVFRKVQQSFETPRAKAQMPTIERLS : 366 caspase-5 : VSWRDRTRGSIFITELITCFQKYSCCC-HLMEIFRKVQKSFEVPQAKAQMPTIERAT : 407 caspase-1 : VSWRHPTMGSVFIGRLIEHMQEYACSC-DVEEIFRKVRFSFEQPDGRAQMPTTERVT : 393 caspase-3 : YSWRNSKDGSWFIQSLCAMLKQYADKLE-FMHILTRVNRKVATE--FESFSFDATFH : 257 caspase-7 : YSWRSPGRGSWFVQALCSILEEHGKDLE-IMQILTRVNDRVARH--FESQSDDPHFH : 283 caspase-6 : YSHRETVNGSWYIQDLCEMLGKYGSSLE-FTELLTLVNRKVSQR--RVDFCKDPSAI : 270 caspase-8 : VSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNK-------DDKK-N : 458 caspase-10: VSFRHVEEGSWYIQSLCNHLKKLVPRHEDILSILTAVNDDVSRR-------VDKQ-G : 452 caspase-2 : AAMRNTKRGSWYIEALAQVFSERACD-MHVADMLVKV-NAL-IKDREGYAPGT-EFH : 410 caspase-9 : VSWRDPKSGSWYVETLDDIFEQWAHS-EDLQSLLLRVANAVSVKGIYKQMPGCFNFL : 407
caspase-4 : MTRYFYLFPGN................ : 377 caspase-5 : LTRDFYLFPGN................ : 418 caspase-1 : LTRCFYLFPGH................ : 404 caspase-3 : AKKQIPCIVSMLTKELYFYH....... : 277 caspase-7 : EKKQIPCVVSMLTKELYFSQ....... : 303 caspase-6 : GKKQVPCFASMLTKKLHFFPKSN.... : 293 caspase-8 : MGKQMPQPTFTLRKKLVFPSD...... : 479 caspase-10: TKKQMPQPAFTLRKKLVFPVPLDALSI : 479 caspase-2 : RCKEMSEYCSTLCRHLYLFPGHPPT.. : 435 caspase-9 : R-KKLFFKTS................. : 416 |