The PCR can be applied in virtually all fields of modern molecular biology:
in the introducing of restriction site sequencesin the construction of cDNA librariesin the isolation and construction of DNA clonesin modifications of DNA sequencesin mutagenesis and analysis of known mutationsin sequencingin detecting of pathogens (viruses, bacteria)The most important applications, in structural molecular biology, are:
The sequencing of DNA, which allows the determining of the
primary structure of proteins
The introducing of mutations, which allows substitution of
aminoacids and searching for changes of the structure and activity of
the macromolecules