The PCR can be applied in virtually all fields of modern molecular biology:

*1*in the introducing of restriction site sequences
*1*in the construction of cDNA libraries
*1*in the isolation and construction of DNA clones
*1*in modifications of DNA sequences
*1*in mutagenesis and analysis of known mutations
*1*in sequencing
*1*in detecting of pathogens (viruses, bacteria)

The most important applications, in structural molecular biology, are:

*1* The sequencing of DNA, which allows the determining of the primary structure of proteins
*1* The introducing of mutations, which allows substitution of aminoacids and searching for changes of the structure and activity of the macromolecules

Go to Sequencing of DNA - determination of the primary structure of proteins
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Dominika Borek
Department of Crystallography
Faculty of Chemistry
Adam Mickiewicz University
Grunwaldzka 6
Poznan, Poland