Last modified 17th April '95 © Birkbeck College 1995
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For a detailed description of lipases, we thank
Sophie Clarke of
Harpreet Singh has also prepared a page on
Some Carboxylic Esterases
Triacylglycerol hydrolase from Candida rugosa fungus.
Three N-acetylglucosamine residues at two sites are
included: two N-linked residues at Asn 351 and one N-linked residue at Asn 314.
Triacylglycerol acylhydrolase from Rhizomucor miehei;
complex with N-hexylphosphanate ethyl ester (C-alpha
atoms only) PIC
Human pancreatic lipase complexed with colipase
Bovine pancreatic phospholipase A2 (phosphatide acyl-hydrolase)
with 2 molecules of 2-methyl-2,4-pentanediol.
For a detailed description of the active site and a proposal for a reaction mechanism,
see Renetseder et al.(1985).
Here is the zymogen ,
which has seven additional residues at the N-terminus (see REMARK 5 and SEQRES records
of the pdb file; N.B. I couldnt seem to find these seven residues though).
Lipases are described by Sophie Clarke of York University in her section on
of triacylglyceride lipase.
- Derewenda, U., Brzozowski, A.M., Lawson, D.M. and Z.S. Derewenda (1992) Catalysis
at the interface: the anatomy of a conformational change in a triglyceride lipase
Biochemistry 31 1532
- Grochulski, P., Li, Y., Schrag, J.D., Bouthillier, F., Smith, P., Harrison, D.,
Rubin, B. and Cygler, M. (1993) Insights into Interfacial Activation from an 'open'
structure of Candida rugosa lipase J. Biol. Chem. 268, 12843
- Renetseder, R., Brunie, S., Dijkstra, B.W., Drenth, J. and Sigler, P.B. (1985)
A comparison of the crystal structures of phospholipase A2 from bovine pancreas and
Crotalus atrox venom J. Biol. Chem. 260 11627
- Van Tilbeurgh, H., Sarda, L., Verger, R. and Cambillau, C. (1992) Structure of the
pancreatic lipase-procolipase complex Nature 359, 159
- Van Tilbeurgh, H., Egloff, M.-P., Martinez, C., Rugani, N.,
Verger, R. and Cambillau, C. (1993) Interfacial activation of the
lipase-procolipase complex by mixed micelles revealed by X-ray crystallography
Nature 362, 814
Here is the index to the Protein Science
Kinemages on Lipases.
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