Ipattern  paragraph style default tab maximum page size arrow size slide show template name circle old arc rectangle old text block 3D viewer polygon group rounded rect new page image text block arc note page tab count token table current item numberaudio clip old templates encapsulated PS current page numbertemplates version string page image back pointer (This file created by Showcase V2.0.3dHF TdÔÿBCõBCå€C±Cõÿÿ¼ ÿÿ ÿÿ €?Momordica (Type I RIP)ÿÿJonas EmsleyÿBernard O'HaraÿTom BlundelTÿB¶CÊB¶C±D&CÊÿÿSEÿÿ ÿÿ €?Isolation, purification, crystallisation & preliminary X-rayÿcharacterisation of toxin from ÿÿ ÿÿ €?Momordica charantiaÿÿ ÿÿ €?ÿÿterisation of tTÿB¼C‚B¼CTD7ÀC‚ÿÿSLÿÿ ÿÿ €?X-ray structure solution achieved using molecular replacementÿwith ricin A-chain as the search modelÿÿy structure solution achieved using molecular replacementÿwith ricin A-chaiÔÿBÀC<BÀC$DÀC<ÿÿéÿÿ ÿÿ €?Refinement proceeding at 2 . 3 Angstrom resolutionÿÿwith ricin A-chaiÔÿCYD ÀCYDD€D Àÿÿ ÿÿ ÿÿ €?RICIN HOMOLOGUESÿÿ 2 . 3 Angstrom resolutionÿÿwith ricin A-chaiÔÿBCÚBCBdCÚÿÿü ÿ@ÿ ÿÿ €?.ÿ.ÿ.ÿ.ÿÿngstrom resolutionÿÿwith ricin A-chaiTÿB¸C¤€B¸C‹D,@C¤€ÿÿn¬ ÿÿ ÿÿ €?Rhombohedral cell (hexagonal setting) , space group R3 , Z = 1ÿcell a = b 131.05 , c = 40.05 Angstrom,ÿÿ ÿÿ €? g = 120ÿÿroup R3 , Z = 1ÿcell a = b 131.05 dÔÿ B,CÜ€B,B¶B|CÜ€ÿÿ_ ÿ@ÿ ÿÿ €?.ÿ.ÿ.ÿ.ÿ.ÿÿroup R3 , Z = 1ÿcell a = b 131.05 Tÿ B\CóB\Cã€D!€Cóÿÿ¼ ÿÿ ÿÿ €?Mistletoe ML-I (ÿÿ ÿÿ €?Viscum albumÿÿ ÿÿ €?, Type II RIP )ÿÿ€?Mistletoe ML-I (ÿÿ ÿÿ €?Viscum albumÿÿ ÿÿ Ôÿ BžCÌBžCD;€CÌÿÿSLÿÿ ÿÿ €?Purification (Berlin group), crystallisation, & preliminary X-rayÿcharacterisation of ML-I A & B chains; SG P6 22 or P6 22ÿa=b=112.14 , c = 311.95 Angstrom, Z=24ÿSequencing (USA) of ML-I A & B-chains at advanced stage revealsÿclose homology to ricinÿÿÿSequencing (USA)Tÿ B”C„€B”CYD"€C„€ÿÿðÿÿ ÿÿ €?Homology-based model of ML-I A-chain produced using ÿCOMPOSER on basis of ricin (A) and pokeweedÿÿ€?Homology-based model of ML-I A-chain produced using ÿCOMPOSER on basis of riciÔÿB”CAB”C)D5ÀCAÿÿë¨ÿÿ ÿÿ €?Conservation of most active site residues; a few mutations near-byÿÿasis of riciÔÿB CB BÐD&Cÿÿ,ÿÿ ÿÿ €?Model of ML-I B-chain produced on basis of ricin B-chainÿÿÿasis of riciÔÿ CUD ÀCUDD€D Àÿÿÿÿ ÿÿ €?RICIN HOMOLOGUESÿÿuced on basis of ricin B-chainÿÿÿasis of riciÔÿDÀC¸DÀC®DÀC¸ÿÿ¦ÿÿ ÿÿ €?1ÿÿLOGUESÿÿuced on basis of ricin B-chainÿÿÿasis of riciÔÿDÀC·€DÀC­€D@C·€ÿÿ ÿÿ ÿÿ €?5ÿÿLOGUESÿÿuced on basis of ricin B-chainÿÿÿasis of ricidÔÿBD ÀBCø€D*D Àÿÿ!ÿÿ ÿÿ €?WHEAT GERM AGGLUTININ HOMOLOGUESÿÿÿn B-chainÿÿÿasis of riciÔÿBCÙBA`BTCÙÿÿ˜#ÿ@ÿ ÿÿ €?.ÿ.ÿ.ÿ.ÿ.ÿÿÿ B-chainÿÿÿasis of riciTÿBCñBCá€Cþ€CñÿÿÃÈ%ÿÿ ÿÿ €?Stinging Nettle Lectin (ÿÿ ÿÿ €?Urtica doicaÿÿ ÿÿ €?)ÿÿ €?Stinging Nettle Lectin (ÿÿ ÿÿ €?Urtica doicaÿÿ ÿÿ TÿB‚CÉ€B‚C±€D1CÉ€ÿÿA'ÿÿ ÿÿ €?Hevein domains occur in different numbers in various plant lectins,ÿagglutinins, chitinases & WIPs ; all homologues of WGAÿÿur in different numbers in various plant lectins,ÿagglutinins, chitinaTÿB€C¥B€CDA@C¥ÿÿ)ÿÿ ÿÿ €?Isolation, purification, crystallisation & preliminary X-ray ÿcharacterisation of rhizome lectin from stinging nettle (Brussels group)ÿÿsation & preliminary X-ray ÿcharacterisation ofÔÿB‚C„B‚C@D1€C„ÿÿ›+ÿÿ ÿÿ €?2nd. crystal form obtained for complex with N'N''N'''-chitotrioseÿcomplex - SG P2 2 2 a=37.69, b=48.97, c=57.32 Angstrom, Z=1ÿnative - SG P2 2 2 a=54.3 , b=62.2 , c=92.4 Angstrom, Z=3ÿÿ57.32 Angstrom, Z=1ÿnative - SG P2 2 2 a=54.3 , b=62.2 , TÿB‚C8B‚BàD7@C8ÿÿÿ-ÿÿ ÿÿ €?Homology modelling based on WGA requires minor insertions &ÿdeletions in its 2 hevein domains and assumptions regarding their ÿrelative dispositionsÿÿns &ÿdeletions in its ÔÿB€BÞB€B®D/@BÞÿÿID/ÿÿ ÿÿ €?Structure determination proceeds using molecular replacementÿÿdeletions in its Ôÿ CeCbCeCQClCbÿÿi1ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its Ôÿ!CwCcCwCRC~Ccÿÿ3ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its Ôÿ"C„€CcC„€CRCˆCcÿÿÀÿ5ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its Ôÿ$CgCKCgC:CnCKÿÿÀ7ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its Ôÿ%C{CKC{C:CCKÿÿ9ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its Ôÿ&C†€CKC†€C:CŠCKÿÿÀ;ÿ ÿ ÿÿ €?1ÿÿetermination proceeds using molecular replacementÿÿdeletions in its dTÿ'BDD ÀBDDD3ÀD Àÿÿ=ÿÿ ÿÿ €?GENE TRIPLICATION & THE ÿÿ ÿÿ €?bÿÿ ÿÿ €? - ÿÿ ÿÿ €?TREFOIL FOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ(AðCþ€AðCÛ€BHCþ€ÿÿ?ÿ@ÿ ÿÿ €?.ÿÿFOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ)AðCÎAðC«BHCÎÿÿNTAÿ@ÿ ÿÿ €?.ÿÿFOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ*AðCAðCtBHCÿÿÀCÿ@ÿ ÿÿ €?.ÿÿFOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ+AðCZAðCBHCZÿÿesEÿ@ÿ ÿÿ €?.ÿÿFOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ,AðBêAðB<BHBêÿÿGÿ@ÿ ÿÿ €?.ÿÿFOLDÿÿ ÿÿ ÿÿ €?bÿÿ ÿÿ €?Ôÿ-B|Cì€B|CÇD<@Cì€ÿÿIÿÿ ÿÿ €?Beta-trefoil fold observed in two sub-domains of ricin B-chain, &ÿalso seen in interleukins - 1 ÿÿ ÿÿ €?a & b ÿÿ ÿÿ €?, acidic and basic fibroblast growthÿfactors and in Kunitz soybean trypsin inhibitorÿÿ €?, acidic and basic fibroblaTÿ.B|CÆ€B|C–€D9@CÆ€ÿÿÂ@Kÿÿ ÿÿ €?ÿAlignment of lectin & isolectin sequences from snowdrop, daffodil, ÿgarlic, and amaryllis reveals triplication (or hextuplication) of a ÿshared sequence motifÿÿ ÿgarTÿ/B|CŠ€B|CeD&€CŠ€ÿÿeMÿÿ ÿÿ €?Concensus . . . . Q X D X N X V X Y . . . . . I WA S N T . . . . ÿcan be aligned with ricin B-chain conserving the tryptophanÿÿ . . . . . I WA S N T . . . . ÿcan Ôÿ0B|C5B|BÚD6C5ÿÿܺOÿÿ ÿÿ €?Gene triplication suggests distant homology to ricin B-chain, and ÿhence probable beta - trefoil fold ; a crude structural model for ÿsnowdrop lectin strengthens this predictionÿÿle beta - trefoil fold ; a crude structural model for ÿsnowdrop lectin strengthens this predictionÿÿTÿ2B|B¢B|AøD:@B¢ÿÿHTQÿÿ ÿÿ €?X-ray diffraction studies are already under way on snowdrop (USA)ÿand amaryllis (ÿÿ ÿÿ €?Hippeastrum sp.) ÿÿ ÿÿ €?(Britain)ÿÿÿand amaryllidÔÿ4AØD€AØD€D?D€ÿÿSÿÿ ÿÿ €?Crystallography Dept . , Birkbeck College , University of London, BRITAINÿÿylliÔÿ5CCû€CCË€CâCû€ÿÿieUÿÿ ÿÿ €?Rex Palmer ÿJasmine HusainÿEdel SweeneyÿTapan ChattopadhyÿÿliÔÿ6BCÀ€BC€D6€CÀ€ÿÿÜÌWÿÿ ÿÿ €?Arbeitsgruppe Phytochemie KAI , Universitat Witten Herdecke, Berlin,ÿ GERMANYÿ Uwe Pfullerÿÿÿ Tÿ7BCŒBB D6ÀCŒÿÿüYÿÿ ÿÿ €?Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Brussel,ÿ BELGIUMÿ Lode Wynsÿ John Lisgartenÿ Remy Lorisÿ Minh Hoa DaoÿÿW.Alton Jones Cell Science Center, Lake Placid, N Y , U S Aÿÿ J. W. Crabbÿÿce Center, Lake Placid, N Y , UÔÿ3C?D@C?D €DD@ÿÿï[ÿÿ ÿÿ €?ACKNOWLEDGEMENTSÿÿ J. W. Crabbÿÿce Center, Lake Placid, N Y , UdÔÿ8B’D ÀB’DD*€D ÀÿÿÀ]ÿÿ ÿÿ €?HUMAN SERUM AMYLOID P - COMPONENTÿÿabbÿÿce Center, Lake Placid, N Y , UÔÿ9B@DÀB@BÆBˆDÀÿÿà_ÿ@ÿ ÿÿ €?.ÿ.ÿ.ÿ.ÿ.ÿ.ÿÿÿce Center, Lake Placid, N Y , UTÿ?BšCò€BšC΀D+Cò€ÿÿaÿÿ ÿÿ €?The pentraxin SAP is a decameric protein composed of two ÿpentameric rings (as is its homologue C-reactive protein)ÿÿÿcameric protein composed of two ÿpentameric rings (Tÿ@BšCÎBšC¶D2ÀCÎÿÿ«cÿÿ ÿÿ €?It binds to DNA of chromatin, fibronectin, glycosaminoglycans,ÿand decorates amyloid fibrils , as in Alzheimer's diseaseÿÿchromatin, fibronectin, glycosaminoglycans,ÿand decorates aTÿAB–C¬B–C”D:@C¬ÿÿD\eÿÿ ÿÿ €?The structure has recently been solved at 2 Angstrom at Birkbeck,ÿrevealing its two calcium binding sites, involved in sugar bindingÿÿed at 2 Angstrom at Birkbeck,ÿrevealing iTÿBB–CJB–CD4ÀCJÿÿgÿÿ ÿÿ €?The N- & C- terminii do not correspond, and the details of the ÿcalcium binding sites are differentÿÿ- & C- terminii do not correspond, and the details of the ÿcalcium biTÿCB˜C‡€B˜C_D;ÀC‡€ÿÿroiÿÿ ÿÿ €?The overall fold is remarkably similar to that of the legume lectins,ÿconcanavalin - A, pea lectin, etc.ÿÿerall fold is remarkably similar to that of the legume lectins,ÿconcanavÔÿEBŽCBŽBÒD>@Cÿÿkÿÿ ÿÿ €?This is the first reported similarity between animal and plant lectinsÿÿanavdÔÿ:CEDÀCEDD€DÀÿÿïmÿÿ ÿÿ €?ACKNOWLEDGEMENTSÿÿted similarity between animal and plant lectinsÿÿanavÔÿ;CMCïCMCãCù€Cïÿÿðoÿÿ ÿÿ €?Birkbeck College S A P Groupÿÿ between animal and plant lectinsÿÿanavTÿ<CˆCÄ€CˆCˆ€C×CÄ€ÿÿèäqÿÿ ÿÿ €?Steve WoodÿHelen WhiteÿJonas EmsleyÿBernard O'HaraÿTom Blundellÿÿÿÿ €?Steve WoodÿHelen WhiteÿJonas EmsleyÿBernard O'HaraÿTom Blundelÿÿÿÿ