(no subject)

CHOISET - Yvan (choiset@nantes.inra.fr)
Sat, 06 Jul 1996 15:15:16 -0700

Dr Graham S Jackson [Biochemistry and Molecular wrote:
> Help required,
> Does anyone know a way of increasing the solubility
> of proteins to assist in obtaining high protein concentrations
> (32mg/ml or 2mM) for NMR ? I've tried things like salt and CHAPS.
> Detergents need
> to be quite mild so as not to disturb tertiary structure.


Try solvent like ethanol in acidic pH sometimes solubility increase
without disturb in secondary and tertiary structure, check with CD for