BioMOO meeting on Quaternary Structure: July 11th

Dr Graham S Jackson [Biochemistry and Molecular (
Thu, 4 Jul 1996 18:02:45 +0100

Help required,
Does anyone know a way of increasing the solubility
of proteins to assist in obtaining high protein concentrations
(32mg/ml or 2mM) for NMR ? I've tried things like salt and CHAPS.
Detergents need
to be quite mild so as not to disturb tertiary structure.