Principles of Protein Structure Assignment 1996
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A Short Primer on Circular Dichroism
CD is a variant of absorption spectroscopy which measures the
difference in absorption of left and right polarised light (in
the ultraviolet) by a medium. Left polarized light will be
absorbed more strongly than right polarized light (or vice-versa)
if the absorbing bond is asymmetric, i.e. if it is chiral. In the
far UV (180 - 250 nm) the CD of a protein is primarily that of
the amide chromophores along the backbone which result from
bonding between the component amino acids. Although the peptide
bond is planar and hence symmetric, there is usually an
asymmetric alpha-carbon on either side, hence the peptide bond
transitions interact to give a CD signal which is very sensitive
to secondary structure.
Here are the far UV CD spectra characteristic of alpha-helix,
beta-sheet and random coil structures.
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Last updated 2nd Apr '96